An Improved Procedure for Binding Parameter Fitting from Scatchard Plot
نویسندگان
چکیده
منابع مشابه
Absurd Trivial Errors in Scatchard Plot Analysis
Several types of widespread errors in performing Scatchard plot analysis resulting in fatal distortions of calculated affinities are surveyed more or less systematically. It appears that graphical analysis of non-linear Scathard plot “by eye” is almost impossible; and, moreover, results calculated by computer programs for affinity analysis often require additional "interpretation". Some of repo...
متن کاملFitting the Three-parameter Weibull Distribution by using Greedy Randomized Adaptive Search Procedure
The Weibull distribution is widely employed in several areas of engineering because it is an extremely flexible distribution with different shapes. Moreover, it can include characteristics of several other distributions. However, successful usage of Weibull distribution depends on estimation accuracy for three parameters of scale, shape and location. This issue shifts the attentions to the requ...
متن کاملScatchard plot and heterogeneity in binding affinity of labeled and unlabeled ligand.
In saturation analysis the Scatchard plot is a generally accepted method for calculation of the affinity constant, K, and the molar concentration, q, of the binder. However, in a system where the K's for the labeled and unlabeled ligand are unequal, a nonlinear plot can be obtained from which incorrect values for K and q may be calculated. This paper mathematically explains how the plot may dev...
متن کاملThe effect of ligand heterogeneity on the Scatchard plot. Particular relevance to lipoprotein binding analysis.
Computer simulations of equilibrium binding studies of a mixture of two labeled ligands binding competitively to a single class of identical and independent sites (receptors) were performed to investigate how ligand heterogeneity affects the observed data in such studies. The simulated data are presented in Scatchard plots. Ligand heterogeneity was generally found to be indistinguishable from t...
متن کاملA New Robust Four Parameter Sine Fitting Procedure
In this paper a new procedure to perform four parameters sine fitting is presented in a closed form, ready for standardization. This new procedure grants convergence of the algorithm, even in those cases where the traditional techniques tend to converge to local minimums of the error function. This new procedure can also be used to compute the initial values for the traditional four parameters ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: YAKUGAKU ZASSHI
سال: 1981
ISSN: 0031-6903,1347-5231
DOI: 10.1248/yakushi1947.101.5_443